Optimized E. coli expression strain LOBSTR eliminates common contaminants from His‐tag purification
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چکیده
منابع مشابه
Optimized E. coli expression strain LOBSTR eliminates common contaminants from His-tag purification.
His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins. We engineered a new E. coli expression strain, LOBSTR (low background strain), which eliminates the most abundant contaminants. LOBSTR is derived from the E. coli BL2...
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ژورنال
عنوان ژورنال: Proteins: Structure, Function, and Bioinformatics
سال: 2013
ISSN: 0887-3585,1097-0134
DOI: 10.1002/prot.24364